The books / Varroa and Colony Health / chapter 6 of 7
When to treat
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Timing a treatment matters as much as choosing one, because the damage that kills a colony over winter is done to bees that have not been born yet. The long-lived winter bees, the ones that must survive four or five months on stored fat reserves alone, are reared from late summer into early autumn.
A mite that feeds on that generation while it develops shortens its lifespan permanently, and no treatment applied afterward can undo it. This is why timing beats speed: killing every mite in October, after the winter bees are already capped, protects almost nothing.
In this game's calendar that puts your real treatment windows early, days zero to forty-five, and again days one hundred forty to one hundred ninety-five, well ahead of the point where the colony commits its next generation to the cold months.
Try this in the game: Treat a hive for varroa.